ABSTRACT
Objective: To compare the inclusion rate of menthol with β-cyclodextrin (β-CD) from different sources.Methods: β-Cyclodextrin-menthol inclusion complexes were prepared by a grinding method.The inclusion compound was verified by thin layer chromatography (TLC), powder X-ray diffraction (PXRD) and gas chromatography (GC).The optimized conditions of PXRD were as follows: the graphite monochromator was Cu Kα, the voltage was 40 kV, the current was 40 mA, the 2θ scanning range was from 3° to 35°, the scanning step was 0.02° and the scanning rate was 5°·min-1.The optimized conditions of GC were as follows: an Agilent DB-wax capillary column, the column temperature was 120°, the inlet temperature was 250°, the detector temperature was 250° and the split ratio was 10∶1.Results: The results of TLC and PXRD proved the formation of inclusion complexes.The inclusion rate of menthol with β-CD from home and abroad was 25% and 47.9%, respectively.Conclusion: The method is simple, accurate and rapid, which can be used to determine the inclusion rate of β-CD from different sources.The results show that the inclusion effect of imported β-CD is better than that of domestic β-CD.
ABSTRACT
Objective: To optimize the preparation of Ginkgo biloba extract-β-cyclodextrin inclusion (GBE50-β-CD inclusion) complex by central composite design and response surface methodology. Methods: Saturated aqueous solution method was used to prepare GBE50-β-CD inclusion complex. The preparation was optimized by central composite design. With the mass ratio of β-CD/GBE50, inclusion time and temperature as the independent variables and with the inclusion rate as the dependent variable, the binomial and linear equations were fitted to the data of overall desirabilities, and the resulting equation was used to produce 3-D response surface graphs, through which optimal formulation was predicted, the best prescription process was chosen to verification test. Results: It showed that the correlation coefficient of second-order quadratic model was higher and the prediction was better (r = 0.966); The optimum conditions for the preparation of GBE50-β-CD inclusion complex were as follows: the mass ratio of β-CD/GBE50 3:1, inclusion time 6 h, inclusion temperature at 60 °C. The deviation between, the result of the best craft verification test and the binomial fitting equation forecast value is 1.07%. Conclusion: Central composite design and response surface methodology is successfully used to optimize the preparation of GBE50-β-CD inclusion complex. The optimized process is reliable, stable, and available for the industrial production.